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. 2020 Apr 15;12(4):1089. doi: 10.3390/nu12041089

Figure 3.

Figure 3

The effect of RCA on NF-kB, iNOS, and COX2 proteins. RAW 264.7 cells were pre-incubated with 5, 10, or 20 μg/mL RCA and then stimulated by 0.5 µg/mL LPS in the presence or absence of RCA. (A) RAW 264.7 cells were stimulated by 0.5 µg/mL LPS for 30 min, 1, 3, 6, and 12 h and then iNOS and COX2 proteins were measured. (B) iNOS and (C) COX2 proteins were determined in RAW 264.7 cells after 12 h-LPS exposure in the presence or absence of RCA. (D) p65 of NF-κB protein was determined in nuclear and cytoplasm of RAW 264.7 cells after 1 h-LPS exposure in the presence or absence of RCA. TBP and β-actin proteins were used as housekeeping proteins in nuclear and cytoplasm, respectively. A different letter indicates a statistically significant difference (p < 0.05).