Table 2.

Identification and quantification of isoflavones of red clover.

Number	RT (min)	Isoflavone Derivatives	Amount (μg/g dw)	[M-H]-m/z
1	27.1	Luteolin 7-O-β-D-glucoside	368.5 ± 14.7	449, 287
2	28.7	unknown tetrahydroxyflavone glucoside	2394.3 ± 28.0	449, 287
3	29.2	unknown tetrahydroxyflavone glucoside	1356.2 ± 18.0	449, 287
4	31.5	Isoquercitrin-6″-O-malonate	1677.9 ± 20.9	551, 303
5	32.3	Pratensein-7-O-β-D-glucoside	682.1 ± 6.3	463, 301
6	33.4	Genistin	12892.3 ± 35.6	433, 271, 153
7	33.9	Hyperoside	3103.0 ± 20.9	465, 303
8	34.3	Isoquercetriin	8940.1 ± 16.6	465, 303
9	35.0	Apigenin-7-O-β-D-glucoside	512.1 ± 1.7	433, 271
10	36.1	Pseudobaptigenin	5809.6 ± 14.0	283
11	37.0	unknown tetrahydroxyflavone glucoside	24560.7 ± 60.7	449, 287
12	38.4	Kaempferol or Luteolin	5022.7 ± 37.8	287
13	38.9	Gemosteom-7-O-β-D-glucoside-6″-O-malonate	17933.8 ± 123.6	519, 271
14	39.3	unknown tetrahydroxyflavone glucoside	2691.2 ± 13.6	449, 287
15	40.2	3-methylquercetin-7-O-β-D-glucoside	7838.7 ± 7.6	479, 317
16	42.7	Kaempferol or Luteolin	12876.9 ± 16.1	287
17	44.3	Pratensein-7-O-β-D-glucoside-6″-malonate	2437.5 ± 8.8	549, 317
18	44.9	Pseudobaptigenin-7-O-β-D-glucoside	2280.1 ± 8.4	445, 283
19	45.5	Kaempferol or Luteolin	2213.7 ± 9.1	287
20	46.7	Glycitein	312.1 ± 15.1	285, 167
21	47.5	Pseudobaptigenin-7-O-β-D-glucoside-6″-O-malonate	2361.1 ± 47.3	445, 283, 137
22	52.2	Formononetin-7-O-β-D-glucoside-6″-O-malonate	2231.1 ± 19.0	517, 269
23	53.7	Calysosin-Glucoside-Malonate	213.7 ± 15.9	533, 285, 137
24	57.0	Prunetin-4′-O-β-D-glucoside-6″-O-malonate	2089.3 ± 38.1	533, 285
25	58.7	Formononetin	497.3 ± 13.6	269, 137
26	60.2	Biochanin A-7-O-β-D-glucoside-6″-O-malonate	3713.8 ± 3.1	533, 285
27	66.8	Biochanin A	1059.8 ± 14.8	285
Total			128069.5 ± 628.9

Values represent means ± standard deviation, RT: retention time, dw: dried weight.