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. 2020 Apr 2;9(4):991. doi: 10.3390/jcm9040991

Figure 5.

Figure 5

Fibroblasts responding to CCL5 from the paracrine culture of ASC/MDA-MB-231 create linear collagen type VI: (A) schematics and immunohistochemistry micrographs of Boyden chamber co-cultures. Top version shows fibroblasts/MDA-MB-231 in Boyden chamber, with fibroblasts beneath. Image below is immunohistochemistry for decellularized collagen VI (meshwork pattern). Bottom version shows ASC/MDA-MB-231, with fibroblasts beneath. Image below is immunohistochemistry for decellularized collagen VI (linear pattern). Scale bar 100 µm. (B) Graph showing qPCR data from cells cultured in Boyden chambers from (B). Error bars represent SD. (* p <0.05). (C) Results from blot against CCL5 of conditioned media taken from the main well of the Boyden co-culture. (D) Schematic illustrating hypothesized axis existing between three key cell types of invasive breast cancer; juxtacrine signaling between ASC and MDA-MB-231, which together produce CCL5, which as a stimulant to the resident fibroblasts for linear collagen VI production.