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. 2020 Mar 28;11(4):367. doi: 10.3390/genes11040367

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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RNAi assays for functional validations. (a,b) Larval feeding of FITC-labelled E. coli. (c) Results of qPCR. Transcription level was dropped in both the genes when blocked by RNAi in comparison to RNAi negative control. (d) Impact of RNAi-based silencing of candidate genes on IVM efficacy against H. contortus L2 larvae. In the control assay, no IVM was used. Silencing of GPCR gene (HCON_00155510) showed similar response as that of control. Silencing of Cyt-P450 gene (HCON_00143950) showed a clear drop in LFI-50 against the increasing concentration of IVM. Experiments were replicated in triplicates. Values represent mean ± SD. *** p < 0.0001.