Figure 11.

Proliferation assay of activated human PBMC cultured with different doses of EVs isolated from different SKOV3 culture medium at 24 h and 120 h. EVs were isolated from the supernatant of SKOV3, either non-treated (NT), subjected to the Photosensitizer (PS) or light (illu) only, or PDT treated (PDT), and compared to PBMC cultivated in their normal culture media (ML10). Results are presented as means of three independent experiments expressed in CPM. Two-way ANOVA test was performed. All quoted p-values are two-sided, with p ≤ 0.001 (**), p ≤ 0.0001 (***) being considered statistically significant for the first and highly significant for the others. n = 3.