Figure 2.

Effects of high insulin and resveratrol on phosphorylated IRS-1 ser³⁰⁷ and ser^636/639 and IRS-1 expression. Whole-cell lysates from L6 myotubes treated without (control, C) or with 100 nM insulin for 24 h (HI) in the absence or the presence of 25 μM resveratrol (RSV) were prepared, resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotted for phosphorylated ser³⁰⁷ and ser^636/639 or total IRS-1. A representative immunoblot is shown (A). The densitometry of the bands, expressed in arbitrary units, was measured using Scion software, and the data were presented as percent of control (B). The values are the mean ± SE of three to five separate experiments (***p < 0.001, **p < 0.01 vs. control; ##p < 0.01 vs. HI).