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. 2020 Apr 15;18(4):213. doi: 10.3390/md18040213

Figure 3.

Figure 3

(A,B) DHE fluorescence staining (A) and HNE immunohistochemistry (B) in CA1 of the vehicle-sham (a), vehicle-IR (b,c), laminarin-sham (d), and laminarin-IR (e,f) groups at one day (b,e) and five days (c,f) after IR. Regarding the vehicle-IR group, DHE fluorescence and HNE immunoreactivity in the stratum pyramidale (SP) are significantly increased at one day post-IR and decreased at five days post-IR. Concerning the laminarin-IR group, DHE fluorescence (asterisk) and HNE immunoreactivity (asterisk) at one day post-IR are significantly lower than those in the vehicle-IR group, and, at five days post-IR, DHE fluorescence and HNE immunoreactivity are maintained. Dihydroethidium, DHE; 4-hydroxy-2-nonenal, HNE; SO, stratum oriens; SR, stratum radiatum. Scale bar = 50 μm. (C,D) Relative immunoreactivity (RI) of DHE fluorescence (C) and HNE immunoreactivity (D) in CA1 pyramidal cells. The bars indicate the mean ± SEM (n = 7/group; ** p < 0.01, *** p < 0.001 versus each sham group, ††† p < 0.001 versus vehicle-IR group).