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. 2020 Apr 23;9(4):1221. doi: 10.3390/jcm9041221

Figure 2.

Figure 2

Serp-1 delivered by chitosan-collagen hydrogel reduces cord damage in rat SCI. (A) Quantification of cord damage area as measured by loss of neurofilament (NF-M) staining for post-SCI treated with 50 µL chitosan-collagen hydrogel alone or 50 µL chitosan-collagen hydrogel with 100 µg Serp-1 at days 7 and 28 after injury. Mean and standard error are presented. Statistics performed by two-way Analysis of Variance (ANOVA) with Fisher’s Least Significant Difference (LSD) post hoc where ** p < 0.01 and *** p < 0.0001. (B) Representative immunohistochemical staining (brown) of NF-M of conditions quantified in panel A. Dotted red area illustrates representative examples of quantified NF-M-negative areas. Boundaries defined are based on continuous tissue outside of the injury area and do not include excessive “spill over” which extend beyond the normal spinal cord boundaries (e.g., day 7 chitosan). Scale bars are 500 µm.