Figure 4.

Serp-1 delivered by chitosan-collagen hydrogel decreases T-cell invasion in rat SCI. (A) Quantification of CD3+ cells in the area of injury in spinal cords of rats post-SCI treated with 50 µL chitosan-collagen hydrogel alone or 50 µL chitosan-collagen hydrogel with 100 µg Serp-1 at days 7 and 28 after injury. Mean and standard error are presented. Statistics performed by Two-Way ANOVA with Fisher’s LSD post-hoc where * p < 0.05 and n.s. is not significant. (B) Representative immunohistochemical staining (brown) of CD3+ cells at day 28 of the same conditions quantified in panel A. Red arrows indicate cells positive for active CD3. Scale bars are 25 µm.