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. 2020 Apr 17;12(4):1125. doi: 10.3390/nu12041125

Figure 2.

Figure 2

Colonic inflammation is limited in immature intestine exposed to MP-WPC compared to extensively heated (EH-)WPC. Relative expression levels of inflammatory cytokines (a) IL-1β, (b) IL-8 and (c) TNF-α in the colon as determined by RT-qPCR. (d) T cells were detected in colon tissue by immunohistochemistry staining for CD3, and (e) staining intensity in the crypt region was quantified by ImageJ analysis. Relative expression levels of (f) CD14 and (g) TLR4 in the colon as determined by RT-qPCR. Values are median ± IQR, * p ≤ 0.05 ** p ≤ 0.01. Scalebar equals 50 µm.