Figure 3.
Innate defense in the colon is influenced by MP-WPC and gestational age. (a) detection of neutrophils by immunohistochemistry in colon tissue, with relative staining intensity of (b) MPO, (c) MMP9 and (d) S100A9 quantified by ImageJ analysis. (e) iALP brush border activity on colon tissue slides was determined with NBT/BCIP conversion and (f) number of piglets with iALP positive brush border was quantified. (g) Total iALP enzyme activity (µg pNPP·mg protein·min−1) determined on protein level in colon tissue homogenates and content. Values are median ± IQR, * p ≤ 0.05 ** p ≤ 0.01. Scalebar equals 50 µm.