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. 2020 Apr 17;12(4):1125. doi: 10.3390/nu12041125

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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MP-WPC affects colonic crypt proliferation & differentiation. (a) Proliferating cells in the colon crypt were identified by immunohistochemistry staining for Ki67 with (b) quantification of the number of Ki67⁺ cells per crypt. (c) Colon crypt depth evaluated by ImageJ analysis. Relative expression levels of stem cell marker (d) OLFM4 and goblet cell marker (e) MUC2 as determined by RT-qPCR and (f) detection of mucus-containing cells in the colon by Periodic-Acid Schiff’s (PAS) staining. Relative expression levels of (g) MUC1 and (h) VIL1 in the colon as determined by RT-qPCR. (i) enterochromaffin cells in de colon were detected by immunohistochemistry staining for serotonin (5HT) and (j) number of 5HT⁺ cells per crypt were quantified. Values are median ± IQR, * p ≤ 0.05 ** p ≤ 0.01 *** p ≤ 0.001. Scalebar equals 50 µm.