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. 2020 Mar 26;12(4):907. doi: 10.3390/nu12040907

Figure 2.

Figure 2

Effect of the intraperitoneal (IP) growth hormone-releasing peptide (GHRP)-6 injection on high-fat diet (HFD) intake. Line graphs represent changes in HFD intake after IP injection of GHRP-6 (A: 80 nmol/kg, B: 400 nmol/kg) or saline in the placebo (Pla; n = 13), 17β-estradiol (E2; n = 14) groups of ovariectomized HFD-fed rats. Data are expressed as means ± SE and were analyzed by two-way repeated-measures ANOVA for each pair-wise comparison among the four groups. There was an interaction of time and group effects in the HFD intake after GHRP-6 injection at a dose of 400 nmol/kg (p Time × Group < 0.05: Pla–GHRP-6 vs. Pla–saline or E2–GHRP-6). Significant differences were observed at 60 min after GHRP-6 injection at a dose of 400 nmol/kg between the Pla–GHRP-6 and the E2–GHRP-6 groups (** p < 0.01), and between the Pla–saline and the Pla–GHRP-6 groups (## p < 0.01). Bar graphs represent the cumulative HFD intake for 2 h after GHRP-6 injection at a dose of 400 nmol/kg in the Pla and E2 groups (C). Two-way repeated-measures ANOVA showed an interaction of injection and group effects in the HFD intake (p Injection × Group < 0.01). Significant differences in HFD intakes were observed between the Pla–saline and the Pla–GHRP groups (## p < 0.01), and between the Pla–GHRP-6 and the E2–GHRP-6 groups (** p < 0.01).