Skip to main content
. 2020 Mar 26;12(4):907. doi: 10.3390/nu12040907

Figure 3.

Figure 3

In vivo effects of 17β-estradiol (E2) replacement on ghrelin and growth hormone secretagogue receptor (GHSR) in the stomach. (A) Representative blots and relative values of ghrelin protein level in the gastric mucosa of HFD-fed rats in the placebo (Pla, n = 9)- and E2 (n = 8)-treated groups. (B) Representative blots and relative values of GHSR protein level in the stomach of the rats in the Pla (n = 11) and E2 (n = 10) groups. (C) Relative values of ghrelin mRNA level in the gastric mucosa of the rats in the Pla (n = 8) and the E2 (n = 8) groups. Data are evaluated as 2−ΔΔCt using β-actin as a housekeeping gene. (D) Plasma acyl ghrelin level after 16-h fasting in the Pla (n = 9) and the E2 (n = 8) groups at 17 weeks of age. Data are expressed as means ± SE and were analyzed by unpaired t-test. * p < 0.05 and ** p < 0.01, differences between E2 and Pla groups.