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. 2020 Apr 22;12(4):1172. doi: 10.3390/nu12041172

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Quantification of food intake measured with a CAFE assay in female (left) and male (right) flies (A). Lifetime fecundity (B) of female flies subjected to normal food (black bars) or on normal food supplemented with 0.1% BAE (red bars) or 0.5% BAE (green bars). Overall physical activities (C) were quantified using the Drosophila Activity Monitor (DAM) system in a 24 h period for females (left) and males (right). Metabolic rates were measured in small cohorts of flies (3 per biological replicate), both in females (D) and males (E) subjected for the indicated times to the different media (control medium, black bars; 0.1% BAE, red bars; 0.5% BAE, green bars). Mean values ± SD are shown. n > 5 for each experiment. * p < 0.05, *** p < 0.001.