Figure 4.

Transcriptional changes of antioxidant-related genes in RB-treated CC4348 cells. The transcript abundance of GPX5 (A), APX1 (B), MSD2 (C), GST1 (D), GST10 (E), LCI7 (F), SOUL1 (G), and SOUL2 (H) and GPX1 (I) at 15, 30, 45, and 60 min after treatment with 1 μM RB in CC4348 were detected, using qRT-PCR. CβLP was used as an internal control to normalize the data. The error bars indicate standard deviation of biological triplicates. The asterisks represent statistical significance between every treated group and the control, which was determined by t-test (n = 3, P < 0.01).