Figure 6.

The response of photosystem to ¹O₂. (A–C) The expression pattern of DEGs encoding light-harvesting proteins in CC4348 (A), the gpx5 mutant (B), and L27 (C) after treated with 1 μM RB determined by RNA-seq. (D–F) The expression pattern of DEGs related to chlorophyll metabolism in CC4348 (D), the gpx5 mutant (E), and L27 (F) after treated with 1 μM RB determined by RNA-seq. The initial RPKM was set as 1 for each strain, separately, to normalize the data. Genes and corresponding RPKM values used in this analysis can be found in Table S6. (G,H) Comparison of the content of chlorophyll (a + b) (G) and maximum efficiency (Fv/Fm) of photosystem II (H) in CC4348, the gpx5 mutant and L27. The same amounts of cells were treated with 1 μM RB for 1 h, and the RB was then washed out, and measurements were taken over a period of 3.5 h. The stage of treatment is shaded with light gray.