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. 2020 Apr 13;18(4):210. doi: 10.3390/md18040210

Figure 2.

Figure 2

Aplykurodin A promotes proteasomal β-catenin degradation. (A) After treatment of HEK293-FL cells with either DMSO or aplykurodin A (20 and 40 μM) in the presence of Wnt3a-CM for 15 h, cytosolic proteins were analyzed by Western blotting with anti-β-catenin antibody. (B) After treatment of HEK293-FL cells with either DMSO or aplykurodin A (20 and 40 μM) in the presence of Wnt3a-CM for 15 h, semi-quantitative RT-PCRs for β-catenin and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were carried out with total RNA. (C) HEK293-FL reporter cells were treated with either DMSO or aplykurodin A (20 μM) and then exposed to MG-132 (10 μM) for 8 h. Cytosolic proteins were analyzed by Western blotting with anti-β-catenin antibody. (A,C) the blots were re-probed with anti-actin antibody. The results are representative of three independent experiments.