Figure 3.

GSK-3β is not required for aplykurodin A-promoted β-catenin degradation. (A) After incubation of HEK293 reporter cells with either DMSO or aplykurodin A (20 and 40 μM) in the presence of 0.75 μM of 6-bromoindirubin-3′-oxime (BIO) for 15 h, FL activity was measured. The results represent the mean ± S.D. of three independent experiments. * p < 0.05 and ** p < 0.01, compared with the BIO-treated control group. (B) After incubation of HEK293 reporter cells with either DMSO or aplykurodin A (20 and 40 μM) in the presence of 0.75 μM of BIO for 15 h, cytosolic proteins were analyzed by Western blotting with anti-β-catenin and anti-β-actin antibodies. The results are representative of three independent experiments.