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. 2020 Apr 13;18(4):210. doi: 10.3390/md18040210

Figure 5.

Figure 5

Aplykurodin A induces apoptosis in SNU475 and Hep3B cells. (A) After incubation of SNU475 and Hep3B cells with either DMSO or aplykurodin A (20 and 40 μM) for 48 h, Annexin V-FITC and propidium iodide (PI) stained cells were analyzed by a Cellometer cytometer. (B) After incubation of SNU475 and Hep3B cells with either DMSO or aplykurodin A (20 and 40 μM) for 48 h, caspase3/7 activity was determined. Details were described in Materials and Methods. The results represent the mean ± S.D. of three independent experiments. * p < 0.01 compared with the DMSO control group. (C) After incubation of SNU475 and Hep3B cells with either DMSO or aplykurodin A (20 and 40 μM) for 48 h, cell extracts were immunoblotted with anti-caspase-3, anti-poly (ADP-ribose) polymerase (PARP) and anti-actin (loading control) antibodies. The results are representative of three independent experiments.