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. 2020 Apr 13;18(4):210. doi: 10.3390/md18040210

Figure 6.

Figure 6

Aplykurodin A promotes autophagy in SNU475 and Hep3B cells. (A) After incubation of SNU475 and Hep3B cells with either DMSO or aplykurodin A (20 and 40 μM) for 48 h, the punctate distribution of GFP-LC3 were analyzed by confocal microscopy. (B) After incubation of SNU475 and Hep3B cells with either DMSO or aplykurodin A (20 and 40 μM) for 48 h, cell extracts were immunoblotted with anti-LC3, p62/SQSTM1, and anti-actin (loading control) antibodies. The results are representative of three independent experiments.