Table 3.
HPLC-DAD identification and quantification of polyphenols and flavonoids from M. charantia extracts.
| Extracts Content (mg/kg) | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Peak | Compound | TR ** (min) |
TR * (min) |
λ max (nm) | H2O | H2O-EtOH 30:70 | MeOH | MeOH/1% HCl | EtOH | EAC | MeOH -EA | MeOH/ HCl-PE |
| 1 | Tannic acid | 2.30 | 2.32 | 280 | 18.74 ± 0.04 | - | - | - | - | - | - | - |
| 2 | Gallic acid | 3.51 | 3.31 | 250 | 30.21 ± 0.23 | - | - | - | - | - | - | - |
| 3 | Chlorogenic acid | 20.86 | 20.93 | 280, 300 | 7.24 ± 1.02 | - | - | 11.35 ± 0.20 | - | - | - | 0.97 ± 0.01 |
| 4 | Epicatechin (-) | 23.56 | 23.68 | 230, 280 | - | - | - | 143.34 ± 0.90 | - | 55.67 ± 0.44 | - | 12.49 ± 0.09 |
| 5 | p-Coumaric acid | 24.10 | 24.10 | 300, 320 | - | 65.88 ± 0.05 | - | 55.12 ± 0.57 | - | - | - | 4.86 ± 0.02 |
| 6 | Daidzein | 26.44 | 26.48 | 280, 320 | - | 9.37 ± 0.04 | 10.16 ± 0.15 | 20.82 ± 0.01 | 10.06 ± 0.52 | - | 3.56 ± 0.02 | 1.83 ± 0.05 |
| 7 | Rutin | 29.70 | 29.71 | 320, 370 | 21.88 ± 0.38 | 38.61 ± 0.06 | 41.56 ± 0.47 | 45.75 ± 0.46 | 40.87 ± 1.05 | - | 20.54 ± 0.10 | 3.83 ± 0.04 |
| 8 | Naringin | 31.51 | 31.72 | 320 | - | - | - | 1.07 ± 0.02 | - | - | - | - |
| 9 | Naringenin | 38.96 | 38.96 | 280, 300 | 29.85 ± 0.53 | - | 38.89 ± 0.36 | 45.44 ± 0.09 | - | - | 19.17 ± 0.38 | 3.96 ± 0.02 |
| 10 | Genistein | 39.11 | 39.20 | 300, 320 | 36.02 ± 0.15 | - | - | 54.77 ± 0.04 | - | - | - | 1.17 ± 0.02 |
** Retention time (TR) mean error for standard references was ± 0,0001–0,2 min. * Retention time (TR) error of mean for compounds was ± 0,0001–0,2 min. - not detected, MeOH: methanol, EtOH: ethanol, EAC: ethyl acetate, PE: petroleum ether.