Figure 2.

Isolation of cells from Wharton’s jelly may be performed with the use of two different methods, namely, explant method or enzymatic digestion. In both cases, the umbilical epithelium and vessels are initially removed, while the residual tissue is mechanically fragmented. The tissue pieces are placed directly on the culture vessel in the explant method, which results in the cell outgrowth on the plastic surface. In the enzymatic method, the tissue pieces are first digested in the enzymatic solution, and after that the cells released from the tissue are centrifuged, suspended in the culture medium, and seeded in the culture vessels.