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. 2020 Mar 27;9(4):915. doi: 10.3390/jcm9040915

Table 1.

UHPLC (Ultra High Performance Liquid Chromatography) gradient program.

Time (min) Gradient (Percentage of Solvent B by Volume)
0.01 10
0.5 10
10.0 55
12.0 90
12.2 10
15.0 10