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. 2020 Apr 17;11(4):439. doi: 10.3390/genes11040439

Table 1.

Comparison of vector production in Sf9 vs HEK293 and with or without NP1 expression.

Vector Helper * Vector Yield
in the Peak Fraction (500 µL)
rAAV2/HBoV1(Sf9) None 1.6 × 109 DRP/µL
rAAV2/HBoV1(Sf9) Bac-HBoV1NP1 5.0 × 109 DRP/ul
rAAV2(Sf9) None 7.5 × 109 DRP/µL
rAAV2/HBoV1(293) pHelper 2.3 × 109 DRP/µL

Note: 200 mL of Sf9 cells at a density of 2 × 106 cells/mL (a total of 4 × 108) and 20 145-mm plates of HEK293 cells (a total of 5 × 108) were infected /transfected for rAAV vector production. Vectors in the peak fraction that has the density of 1.40 g/mL in CsCl were quantified after dialyzed. * Helper: other than rep/cap trans complementary.