Figure 2.

Effect of A009 extracts on the induction of apoptosis in vitro, in lung cancer cells. The lung carcinoma cell lines A549 (A-B) and H1650 (A-B) were treated for 24–48 h with 2 batches of A009 (L4 and L3), at dilutions 1; 500, 1: 250, 1: 100, or with hydroxytytosol (HyT) at the same concentration present in the dilutions of A009. The effects on induction of apoptosis was evaluated by flow cytometry, following labeling with Propidium Iodide (PI) and AnnexinV. Results are shown as mean ± SEM, One Way ANOVA, * p < 0.5, ** p < 0.01, *** p < 0.001, **** p < 0.0001. NT (untreated); EtOH (ethanol vehicle used to dissolve hydroxytyrosol).