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. 2020 Mar 26;12(4):903. doi: 10.3390/nu12040903

Figure 5.

Figure 5

Effect of the A009 extracts on the CXCR4/CXCL12 axis in lung cancer cells in vitro. The A549 and H1650 lung cancer cells were treated for 6 h with 2 different batches of A009 (L4 and L3), at the dilutions 1: 250 or with hydroxytytosol (HyT) at the same concentration present in the dilutions of A009. Protein secretion was stimulated with a cocktail containing BrefeldinA and Monensin + Ionomycin and Forbol 12-meristate, 13-acetate. After 6 h of stimulation, cells were analyzed for CXCR4 (A), CXCL12 (B) expression, by flow cytometry. Representative dot plots for CXCR4 surface detection are showed (A). Flow data were analyzed using FlowLogic software. Results are shown as mean ± SEM, One Way ANOVA, * p < 0.5, ** p < 0.01. NT (untreated); EtOH (ethanol vehicle used to dissolve hydroxytyrosol).