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. 2020 Apr 22;12(4):1175. doi: 10.3390/nu12041175

Figure 1.

Figure 1

Effects of treatments on cell integrity in Caco-2/15 cells. Integrity of the cell monolayer was evaluated by cell viability, differentiation, and tight junction assays using fully differentiated Caco-2/15 cells. Different doses (0.5, 1, and 2 mg/mL) of purified glycomacropeptide (GMP) powder were added to Caco-2/15 cells 18 h before incubation with Fe/Asc (200 µM/2 mM) for 6 h at 37 °C for the (A) MTT assay. A dose of 2 mg/mL was selected for the assessment of (B) villin and (C) occludin protein expression as well as (D) transepithelial electrical resistance (TEER). Results represent the means ± SEM of n = 3 independent experiments in triplicate.