Table 3.
Effect of the ethanol extracts of 6 different insect species on blood coagulation.
| Extract/Chemicals | Concentration (mg/mL) | Anti-Coagulation Activity (Multiplication of Control) | ||
|---|---|---|---|---|
| TT 1 | PT 2 | aPTT 3 | ||
| DMSO | - | 1.00 ± 0.07 bc | 1.00 ± 0.00a | 1.00 ± 0.01 b |
| Aspirin | 1.5 | 1.64 ± 0.03 f | 1.39 ± 0.02e | 1.37 ± 0.03 d |
| 5.0 | >15.0 | >15.0 | >15.0 | |
| AD | 5.0 | 1.10 ± 0.01 d | 1.02 ± 0.02 ab | 1.11 ± 0.10 c |
| TM | 5.0 | 0.90 ± 0.00 a | 1.13 ± 0.03 c | 0.93 ± 0.01 ab |
| PB | 5.0 | 1.04 ± 0.03 c | 1.20 ± 0.01 d | 0.95 ± 0.01 ab |
| GB | 5.0 | 0.96 ± 0.00 b | 1.18 ± 0.00 d | 0.90 ± 0.02 a |
| TE | 5.0 | 1.31 ± 0.01 e | 1.05 ± 0.02 b | 1.75 ± 0.02 e |
| AM | 5.0 | 1.04 ± 0.04 c | 1.03 ± 0.04 ab | 0.96 ± 0.00 ab |
Anti-coagulation activity was calculated on the clotting time of a given sample divided by the clotting time of the solvent control in blood coagulation assays. The thrombin (TT1), prothrombin (PT2) and activated partial thromboplastin times (aPTT3) of the solvent control (DMSO) were 31.5 s, 20.5 s and 80.9 s, respectively. Data are means ± SD of triplicate determinations. Different superscripts within a column indicate statistically significant differences (p < 0.05).