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. 2020 Apr 21;11(4):435. doi: 10.3390/mi11040435

Figure 5.

Figure 5

Comparison of the amplification and dissolution curves of H7N9 and pGEM-3Zf (+)1 with those obtained on a commercial instrument. Curves obtained by the real-time PCR amplification system under the same conditions are shown in the upper left of each figure. (a): The amplification curves of H7N9 from the homemade system are shown. (b): The melt curve analysis of the H7N9 obtained from the homemade system. The first derivative change in fluorescence intensity as a function of temperature is shown. (c): The amplification curves of H7N9 from the homemade system are shown. (d): The melt curve analysis of the H7N9 obtained from the homemade system. The first derivative change in fluorescence intensity as a function of temperature is shown.