Figure 6.

Fluorescent images depicting the flow of fluorescent bead through the semi-always-closed single-layer valve. (A) 0.2 μm fluorescent beads diffusing through the valve side opening. (B) 10 μm fluorescent beads getting trapped in the microfluidic valve, we can see that the fluid continues to flow due to the shade of a flowing bead (red circle). (C) 5 μm fluorescent bead initially getting trapped before actuation of the single layer valve. (D) 5 μm fluorescent bead passing through the single layer valve once the side wall was deflected.