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. Author manuscript; available in PMC: 2020 May 17.
Published in final edited form as: Cell Rep. 2019 Apr 30;27(5):1364–1375.e5. doi: 10.1016/j.celrep.2019.04.013

Figure 1. Selective Ablation of MCU in Brown Fat.

Figure 1.

(A) Effect of brown fat-specific Cre recombinase expression on uniporter protein levels on the Mcu[fl/fl] background.

(B) Left: representative Ca2+ uptake traces in isolated brown fat mitochondria energized with glycerol-3-phosphate (G3P) + rotenone in the presence of guanosine diphosphate (GDP). Right: quantification of Ca2+ uptake rates (n = 4).

(C) Oxygen consumption measurements of isolated brown fat mitochondria in a well stirred cuvette at room temperature. Mitochondria, G3P, and GDP were added at indicated time points.

(D) Quantification of oxygen consumption rates (n = 4).

Results are reported as mean + SEM. Statistical significance is indicated as **p < 0.01 (Student’s t test).