(A) Core body temperature of mice transferred from room temperature to 4°C (n = 5–6 per group). Full body temperature data over time is presented in Figure S3A.
(B) Core body temperature of mice transferred to 4°C following 1 week habituation to 30°C (n = 7 per group). Full body temperature data over time is presented in Figure S3B.
(C and D) Body mass of male (C) and female (D) —Cre and +Cre animals fed high-fat diet. Full body mass data over time is presented in Figures S3C and S3D.
(E) Brown fat pyruvate dehydrogenase (PDH) phosphorylation in —Cre or +Cre mice with or without ad libitum access to food at room temperature for 6 h. Animals were starved for 12 h overnight prior to the experiment.
(F) Brown fat pyruvate dehydrogenase (PDH) phosphorylation in —Cre or +Cre mice fasted at room temperature or 4°C for 6 h.
Results are reported as mean + SEM.