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. Author manuscript; available in PMC: 2020 May 17.
Published in final edited form as: Nat Plants. 2020 Apr 13;6(5):522–532. doi: 10.1038/s41477-020-0633-3

Extended Data Fig. 8. Mutations in the 5’ UTR hairpin affect PIF7-MYC protein accumulation.

Extended Data Fig. 8

Western blots of PIF7-MYC protein of independent PIF7::PIF7-MYC transgenic lines harbouring wild-type (WT), 3’ and 5’ disrupted (d3, d5), reconstituted (r) and stabilised (st) hairpin sequences. Seedlings were grown in LD at constant 17°C or with a 27°C midday. Actin levels are shown as loading control. Blots were used for quantifications shown in Fig. 4j-l and Supplementary Figure 9b. The experiment was repeated once with similar results.