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Work flow and experimental design. After excision, both fresh tissue and fluorescent images were captured before and after 10-min of incubation with 6qcNIR probe. Specimen was then cut transversely into bread-loaf sections and underwent Puzzle-fit analysis to correlate the fluorescence with histology. After a 15-min training session, all fresh tissue and fluorescent images were read by blinded dermatologists in the Reader Study.
