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. Author manuscript; available in PMC: 2021 May 1.

Published in final edited form as: J Mol Biol. 2020 Apr 1;432(10):3289–3308. doi: 10.1016/j.jmb.2020.03.030

Table 1.

Mre11 DNA binding constants

	ssDNA Binding
Pf Mre11 Mutant	MR^NBD		Mre11^ND
	K_D (μM)	n	K_D (μM)	n
WT	0.18 ± 0.01	2.1 ± 0.1	0.13 ± 0.01	2.1 ± 0.1
Y187C	0.21 ± 0.01	1.9 ± 0.1	0.24 ± 0.01	1.8 ± 0.1
H52S	0.17 ± 0.01	2.1 ± 0.1	0.17 ± 0.01	1.9 ± 0.1
Y187F	0.19 ± 0.01	2.0 ± 0.1	0.11 ± 0.01	2.1 ± 0.1

	dsDNA Binding
Pf Mre11 Mutant	MR^NBD		Mre11^ND
	K_D (μM)	n	K_D (μM)	n
WT	1.41 ± 0.11	0.9 ± 0.1	1.30 ± 0.04	1.5 ± 0.1
Y187C	1.80 ± 0.31	0.8 ± 0.1	1.98 ± 0.05	1.5 ± 0.1
H52S	4.17 ± 0.44	1.0 ± 0.1	3.17 ± 0.17	1.6 ± 0.1
Y187F	1.32 ± 0.09	0.9 ± 0.1	0.71 ± 0.01	2.4 ± 0.1

n is the Hill coefficient.

Values are the average ± the standard deviation of at least three replicates.