Table 2.

Thermodynamics of dsDNA melting in the presence and absence of Mre11^ND

	Rpa	Tm (°C)b	ΔH (kJ moi−1)b	ΔS (kJ mol−1 K−1)c	ΔG (kJ moi−1)d	ΔΔGe	ΔΔGf
apo	0.995	52.4 ± 0.7	−98.1 ± 6.2	−0.36 ± 0.03	18.1
WT	0.998	58.7 ± 0.4	−117.7 ± 5.3	−0.43 ± 0.03	21.8	−3.7
Y187C	0.998	57.5 ± 0.4	−103.8 ± 4.4	−0.38 ± 0.02	19.2	−1.1	2.6
H52S	0.993	54.9 ± 0.9	−106.3 ± 9.0	−0.39 ± 0.05	19.7	−1.5	2.1
Y187F	0.997	59.2 ± 0.7	−117.7 ± 6.6	−0.43 ± 0.03	21.9	−3.7	0.0

^aPearson correlation coefficient

^bErrors are from the covariance of the fits.

^cCalculated with Eq. 5 of Petruska and Goodman [41]. Errors propagated from ΔH.

^dCalculated with ΔG = ΔH – TΔS at 50 °C, which is the temperature used for NMR experiments.

^eΔG_{Mre11ND, bound} - ΔG_apo

^fΔG_wt - ΔG_mutant