Figure S1.

Summary of the 10X scRNA and scVDJ Sequencing of 12 Convalescent Patients’ B Cells, Related to Figure 1

(A) Summary of the 10X scRNA and scVDJ sequencing of 12 convalescent patients’ B cells. Patient 1-9 used a MACS-based negative selection for B cell enrichment from PBMC. Patient 10-12 used a MACS-based CD27⁺ selection for memory B cell enrichment from PBMC. Cells are assigned to the same clonotype if they have identical heavy and light chain CDR3 DNA sequences. (B) Characteristics of the selected mAbs identified from the 12 patients. mAbs are selected from clonotypes that contain IgG1-presenting memory B cells. (C) Characteristics of the neutralizing mAb BD-23. (D) t-SNE plot of patient 4’s scRNA-seq result. Only productive Heavy-Light chain paired cells were shown. Cells are colored based on cell types. (E) t-SNE plot of patient 11’s scRNA-seq result. (F) Top 25 most enriched clonotypes of patient 4. The clonotype containing BD-23 is labeled. (G) Ig class distribution of patient 4’s clonotypes. (H) Top 25 most enriched clonotypes of patient 11. (I) Ig class distribution of patient 11’s clonotypes.