Figure S4.

Binding Specificity and Neutralizing Abilities of the Non-potent Neutralizing mAbs, Related to Figure 2

(A) Neutralization potency measured by a SARS-CoV-2 spike-pseudotyped VSV neutralization assay. Data for each mAb were obtained from a representative neutralization experiment, which contains three replicates. Data are represented as mean ± SD. IC₅₀ and IC₈₀ were calculated by fitting a four-parameter logistic curve. (B) Characteristics of the neutralizing mAbs. K_D is measured using SPR with a 1:1 binding model targeting biotinylated RBD protein. The somatic hypermutation rate (SHM) is calculated from mutated DNA sequences of the heavy- chain variable regions (V, D, and J regions) using Igblast.