Table 1.

Summary of the 10X scRNA and scVDJ Sequencing of Antigen-Binding B Cells

	Enrichment Antigen	PBMC Status	VJ Paired Cells	Naive B Cells	Memory B Cells	Exhausted B Cells	Non-B Cells	IgG1 Clonotypes
Batch 1	S protein	fresh	14,895	5,417	6,333	1,680	1,465	2,598
Batch 2	S protein	frozen	6,962	1,051	1,161	362	4,388	883
Batch 3	RBD protein	fresh	6,000	1,668	2,603	498	1,231	812
Batch 4	S1 (RBD-)	fresh	11,536	3,202	6,413	1,710	211	3,093
Batch 5	RBD protein	fresh	8,020	3,532	3,302	823	363	916
Batch 6	RBD protein	fresh	2,925	1,177	1,353	260	135	256
Total			50,338	16,047	21,165	5,333	7,793	8,558

The experimental details for each sequencing batch. Enrichment antigen refers to the antigen used for the MACS pull-down. Batch 4 was performed on samples that have RBD-binding B cells filtered. PBMC status refers to whether the PBMC used were previously frozen. VJ paired cells refer to cells that both full-length heavy- and light-chain variable regions were detected. Cells are assigned to the same clonotype if they have identical heavy- and light-chain CDR3 DNA sequences. See also Figure S2.