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Protein expression of MMP-2 and MMP-9 in the cells overexpressing BTK. Plasmid containing negative control (NC) or overexpressing BTK (OE) sequence were transfected into DU145 cell or PC3 cell as described in the methods. Cells were then treated with 5µM Ibrutinib followed by immunoblotting as described in the methods. (A) Protein of MMP-2 and MMP-9 in the DU145 cells. (B) Protein of MMP-2 and MMP-9 in the PC3 cells.
