Figure 2.

Overexpression of miR-153-5p enhanced the pro-apoptotic effect of PTX in PTX-resistant TNBC cells. MDA-MB-231/PTX cells were treated with PTX (20 nM) or/and miR-153-5p agonist (10 nM) for 72 h. (A, B) Apoptotic cells were detected with Annexin V and PI double staining. (C) Expression levels of Bax, Bcl-2 and cleaved caspase 3 in MDA-MB-231/PTX cells were detected with Western blotting. (D, E, F) The relative expressions of Bax, Bcl-2 and cleaved caspase 3 in cells were quantified via normalizing to β-actin. *P<0.05, **P<0.01 compared with control group; ^##P<0.01 compared with PTX_20 nM group.

Abbreviations: PI, propidium iodide; PTX, paclitaxel; Bax, BCL2-associated X; Bcl-2, B-cell lymphoma 2; TNBC, triple-negative breast cancers.