Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 May 13;12:3419–3431. doi: 10.2147/CMAR.S240268

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2020 Wang et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

TSN up-regulates miR-608 level and negatively regulates Notch1 and Notch2 expressions. (A) The relative miR-608 levels in U-138MG or U-251MG cells after TSN treatment (0nM, 50nM, 100nM, 150nM), as determined using qRT-PCR. (B) The protein levels of Notch1 and Notch2 in U-138MG or U-251MG after TSN treatment (0nM, 50nM, 100nM, 150nM), as determined using Western blotting. Relatively quantitative results were determined by Image J and shown as histogram. (C) Intersection analysis of miR-608 and Notch1/Notch2 using prediction software of TargetScan. The predicted seed-recognition sites in the 3ʹ-UTR of Notch1 and Notch2 sequence and the corresponding miR-608 sequence was depicted. (D) The relative miR-608 levels in HEK293 cells after transfection with miR-NC or miR-608, as determined using qRT-PCR. (E) The relative luciferase activity of the Notch1 and Notch2 3ʹ-UTR reporter plasmids was assayed in HEK293 cells after transfection with miR-NC, miR-608, NC-inh or miR-608-inh. The mutant Notch1 and Notch2 3ʹ-UTR reporters were also used as controls. (F) The protein levels of Notch1 and Notch2 in U-138MG or U-251MG cells after transfection with miR-NC or miR-608, as determined using Western blotting. Relatively quantitative results were determined by Image J and shown as histogram. (G) The protein levels of Notch1 and Notch2 in U-138MG cells after treated with TSN, TSN+NC-inh or TSN+miR-608-inh, as determined using Western blotting. Relatively quantitative results were determined by ImageJ and shown as histogram. The data are expressed as the mean ± SEM, ^**/##P < 0.01.