Figure 4.

MK2206 enhances CDDP-induced apoptosis through the suppression of Akt pathway activity in testicular cancer cell lines. (A) Cells were exposed to MK2206 (600 nM) alone for the indicated times. “0 hours” refers to the time for addition of reagents. *P < .05 vs. 0 hours. (B) Cells were treated with CDDP at a concentration corresponding to IC30 (130 μM), with or without addition of 600 nM of MK2206 for the indicated times, and the abundance of indicated protein was examined using Western blot. *P < 0.05 vs. 0 hours. (C) Cells were harvested after treatment with or without CDDP at indicated doses corresponding to IC30 for each cell line in the presence or absence of 600 nM of MK2206 for 12 hours. Bars represent SD. *P < 0.05 vs. DMSO or CDDP.