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. 2020 May 15;13(7):100782. doi: 10.1016/j.tranon.2020.100782

Figure 2.

Figure 2

VSV can infect BOECs and transfer infection to NSCLC cells in vitro. A) An equal number of VSV-infected BOECs were co-cultured with H2009 cells and cell viability measured after 72 hours by trypan blue exclusion (Note: BOECs are not adherent to wells in absence of collagen-coating and are washed away prior to counting). Cell viability is normalized to control (PBS-treated BOECs co-cultured with H2009 cells). *indicates statistical significance (P < .002) compared to control. B) Co-culture of H2009 cells to VSV-hIFNβ-infected hBOECs (MOI =10) was done at varying ratios of H2009:hBOECs as indicated. Cell viability was normalized to H2009 cells treated with uninfected hBOEC cells. *indicates statistical significance (P < .03) compared to control. C) Viral titer was determined from medium harvested from a ratio of H2009 cells to VSV-hIFNβ infected hBOECs. Viral titers were determined using viral plaque assay.