Table 2.
AP and CaTr biomarkers Simulated by the Paci2020 hiPSC-CM Model at 37°C
| Biomarker (Reference) | Experimental Value (Mean ± SD) | Simulated Value |
|---|---|---|
| APA (mV) (50) | 104 ± 6 | 102 |
| MDP (mV) (50) | −75.6 ± 6.6 | −74.9 |
| AP CL (ms) (50) | 1700 ± 548 | 1712 |
| dV/dtmax (V/s) (50) | 27.8 ± 26.3 | 20.5 |
| APD10 (ms) (50) | 74.1 ± 26.3 | 87.0 |
| APD30 (ms) (50) | 180 ± 59 | 224 |
| APD90 (ms) (50) | 415 ± 119 | 390 |
| AP Tri (−) (50) | 2.5 ± 1.1 | 2.8 |
| CaTr DURATION (ms) (16) | 805 ± 188 | 691 |
| CaTr tRise10, 50 (ms) (16) | 82.9 ± 50.5 | 54.9 |
| CaTr tRise10, 90 (ms) (16) | 167 ± 70 | 118 |
| CaTr tRise10, peak (ms) (16) | 270 ± 108 | 184 |
| CaTr tDecay90, 10 (ms) (16) | 410 ± 100 | 341 |
| CaTr CL (ms) (16) | 1654 ± 630 | 1712 |
AP and CaTr biomarkers are from (16). Both AP and CaTr biomarkers were recorded at 37°C. AP biomarkers (patch-clamp): APA, AP amplitude; MDP, maximal diastolic potential; CL, cycle length; dV/dtmax, maximal upstroke velocity; APD10, APD30, APD90, AP duration at 10, 30, and 90% of repolarization; AP triangulation (AP Tri) computed as the ratio between APD30–APD40 and APD70–APD80; CaTr DURATION, CaTr rise time from 10 to 50% (CaTr tRise10, 50), 90% (CaTr tRise10, 90), and to CaTr peak (CaTr tRise10, peak), decay time from 90 to 10% (CaTr tDecay90, 10), and CaTr rate (CaTr CL).