Table 1.
Equilibrium and Rate Constants from Lineshape Analysis
| Peptide | Temperature (°C) | 1D, 19F |
2D, 1H-15N HSQC |
||||
|---|---|---|---|---|---|---|---|
| KD (μM) | kon (108 M−1 s−1) | koff (104 s−1) | KD (μM) | kon (108 M−1 s−1) | koff (104 s−1) | ||
| PepS1a | 5 | 1100 | 0.18 | 1.9 | 1100 | 0.30 | 3.4 |
| PepS2 | 45 | 150 ± 10 | 1.5 ± 0.1 | 2.2 ± 0.1 | 150 ± 10 | 1.5 ± 0.3 | 2.2 ± 0.8 |
| PepS2b | 45 | – | – | – | 70 ± 10 | 1.2 ± 0.3 | 0.8 ± 0.2 |
| PepS3 | 45 | 1200 ± 100 | 0.5 ± 0.2 | 6 ± 3 | 1200 ± 100 | 0.081 ± 0.005 | 1.0 ± 0.1 |
| PepS4 | 25 | 60 ± 10 | 0.6 ± 0.1 | 0.29 ± 0.02 | 60 ± 10 | 0.37 ± 0.08 | 0.19 ± 0.01 |
| PepS4 | 45 | 210 ± 30 | 1.1 ± 0.1 | 2.3 ± 0.3 | 230 ± 20 | 0.46 ± 0.04 | 1.1 ± 0.1 |
a
Uncertainty not reported because the measurement was made once.
b
Non-fluorine-labeled SH3 T22G.