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. 2020 Apr 15;118(10):2612–2620. doi: 10.1016/j.bpj.2020.03.032

Figure 3.

Figure 3

hKv2.1-T377A and hKv3.1-T400A produced K+ selective channels. (a) and (b) show representative current recordings for hKv2.1 and its T377A mutation (a) and for hKv3.1 and its T400A mutation (b), respectively. Currents were elicited with the pulse protocol shown at the top, and the horizontal bar at the start of the recordings indicates the zero-current level. (c) and (d) show current (I) versus voltage curves (IV curves) that were obtained by plotting the normalized amplitude of the deactivating currents from recordings shown in (a) and (b) (normalized to the current amplitude at +60 mV) as a function of repolarizing potential. The IV curves of both hKv2.1-T377A and hKv3.1-T400A (solid circles) were comparable to those of the WT channels (open circles). WT and mutant channels displayed a similar reversal potential, i.e., the potential where the current is 0.