Table 4.

ITC Data

Ligand	n	Kd (μM)	$\Delta {\text{G}}_{\text{Bind}}^0$ (kcal ⋅ mol−1)	$\Delta {\text{H}}_{\text{Bind}}^0$ (kcal ⋅ mol−1)	$-\text{T}\Delta {\text{S}}_{\text{Bind}}^0$ (kcal ⋅ mol−1)	$\Delta {\text{S}}_{\text{Bind}}^0$ (cal ⋅ mol−1 ⋅ K−1)
wt	1.19	100 ± 15	−5.4 ± 0.8	−9.5 ± 1.2	4.2 ± 1.2	−14 ± 4.0
Y116A	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
H117A/D118A/H119A	n.d.	n.d.	n.d.	n.d.	n.d.	n.d.
G120E	1.23	70 ± 9	−5.7 ± 0.7	−8.4 ± 0.6	2.7 ± 0.6	−8.9 ± 2.1
G125E	1.31	210 ± 90	−5.0 ± 2.0	−7.5 ± 2.6	2.5 ± 2.7	−8.4 ± 9.2

T = 298 K. n.d., not determined.