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. 2020 Apr 8;118(10):2502–2512. doi: 10.1016/j.bpj.2020.03.026

Table 2.

List of High-Occupancy Contacts and Side-Chain Entropy Changes


H-Bond
Nonpolar Contact
nSH3 PRM Occupancy nSH3 PRM Occupancy
nSH3:PRMcAbl E166n (−1.35) R767 (−3.48) 0.987 F141R (−2.82) K760 (−0.45) 0.930
Y1863 (−0.59) P761 (+0.02) 0.939 F141R (−2.82) P761 (+0.02) 0.769
F143R (+0.00) L763 (−0.63) 0.928
Q168n (−0.58) P764 (+0.24) 0.949
W169n (−2.97) L763 (−0.63) 0.840
P1833 (−0.66) L763 (−0.63) 0.876
P1853 (+0.05) P764 (+0.24) 0.772
Y1863 (−0.59) P761 (+0.02) 0.876
nSH3:PRMNS1A D142R (+0.78) R211 (−1.26) 0.839 F141R (−2.92) R211 (−1.26) 0.900
D147R (−0.91) K217 (−1.41) 0.839 F141R (−2.92) P212 (−0.03) 0.785
D150R (−0.12) K217 (−1.41) 0.943 F143R (+0.06) L214 (−0.84) 0.955
E166n (−0.89) R220 (−4.50) 0.968 Q168n (−0.56) P215 (+0.93) 0.970
Y1863 (−0.94) P212 (−0.03) 0.978 W169n (−3.15) L214 (−0.84) 0.897
W169n (−3.15) K217 (−1.41) 0.951
P1833 (−0.75) L214 (−0.84) 0.886
P1853 (+0.04) P215 (+0.93) 0.870
Y1863 (−0.94) R211 (−1.26) 0.831
Y1863 (−0.94) P212 (−0.03) 0.926
Y1863 (−0.94) L214 (−0.84) 0.754
nSH3:PRMNS1B D142R (+0.23) R211 (−2.11) 0.721 F141R (−3.00) R211 (−2.11) 0.928
D147R (−1.57) K217 (−4.47) 0.933 F141R (−3.00) P212 (+0.08) 0.746
E149R (−0.37) K217 (−4.47) 0.825 F143R (−0.14) L214 (−1.00) 0.949
E149R (−0.37) R220 (−1.71) 0.799 Q168n (−0.65) P215 (+0.94) 0.939
D150R (−0.18) K217 (−4.47) 0.985 W169n (−2.83) L214 (−1.00) 0.872
E166n (−1.10) R220 (−1.71) 0.783 W169n (−2.83) K217 (−4.47) 0.920
Y1863 (−1.29) P212 (+0.08) 0.979 P1833 (−0.71) L214 (−1.00) 0.914
P1853 (+0.06) P215 (+0.94) 0.826
Y1863 (−1.29) R211 (−2.11) 0.858
Y1863 (−1.29) P212 (+0.08) 0.926
Y1863 (−1.29) L214 (−1.00) 0.741

Hydrogen bond (H-Bond) includes salt bridges. Residues of nSH3 are marked with superscripts with the corresponding subdomains (3: 310 helix; R: RT loop; and n: n-Src loop). Side-chain entropy change of each residue upon complex formation (in cal/(molK)) is in parentheses.